KANEKA CORPORATION

KANEKA New breeding technology. Taking agriculture beyond current limits to a whole new level.

KANEKA iPB _{(in planta particle bombardment)} technology

Greatly expedites crop breeding.
Germplasm independent (without complicated tissue culture)
Revolutionizes genome editing.

Hamada et al 2017, 2018, Imai et al 2020

Licensed to seed companies.
Granted highly recognized awards: JSPCMB Award for technical advance 2018,
Award from Agriculture, Forestry and Fisheries Research Council of Japan 2018, etc.

Introduction of iPB technology

Pure-series _{(agrobacterium mediated Tissue culture/transformation technology )}

Gene transfer to cereals by Agrobacterium tumefaciens developed by Former JT (Japan Tobacco Inc.) is the global standard.

Five major cereals can be transformed at frequencies higher by a digit or two than the numbers reported in many publications.

JT/KANEKA Technology	Crop	Variety	Independent events/explant
JT/KANEKA Technology	Crop	Variety	JT/KANEKA	Publications
PureIntro™	Rice	Japonica Indica	15.0 5.0	0.3 0.1
PureUpgrade™	Maize	A188 W117 H99 B104 B73	0.5 0.3 0.2 0.1 0.0	0.0 - 0.1
PureWheat™	Wheat	Fielder Intermediates Recalcitrant elites	0.7 0.1 - 0.3 0.0	0.05
PureBarley™	Barley	Golden Promise	4.0	0.2
PureSorghum™	Sorghum	Tx430	0.2	0.02

CB1 and WOX5b technologies can further elevate efficiency and widen transformable genotypes.

JT/KANEKA Technology	Crop	Variety	Independent events/explant
JT/KANEKA Technology	Crop	Variety	JT/KANEKA	Publications
PureUpgrade™	Maize	A188 B73	0.5 0.0	0.0 - 0.1
PureUpgrade™ + CB1		A188 B73	0.7 0.03
PureUpgrade™ + WOX5b		B73	0.2 - 0.9
PureWheat™	Wheat	Fielder Intermediates Recalcitrant elites	0.7 0.1 - 0.3 0.0	0.05
PureWheat™ + CB1	Wheat	Fielder Intermediates Recalcitrant elites	0.9 0.3 - 0.5 0.1 - 0.3	0.05